NETWORKS IN Drosophila melanogaster

Table of Contents

Preface, Acknowledgements & Statement of Support


1.) Introduction
1.1 Diversifying Networks in Development and Evolution
1.2 Early Mesoderm Development
1.3 Muscle Formation
1.4 Muscle Attachment
1.5 Early CNS Development
1.6 Neuron and Glia Cell Specification
1.7 Motorneuron Outgrowth, Target Finding and Innervation
1.8 Objective of this Thesis

2.) Characterisation of the Abdominal Muscle Pattern
2.1 Introduction
2.2 A Map of the Larval Muscle Pattern from A1 to A9
2.3 Innervation and Classification of the Muscles in A8/9
2.4 Discussion

3.) Characterisation of Motorneurons for Muscles in A8 and A9
3.1 Introduction: Motorneurons of the Reference Segment
3.2 Motorneurons of A8/9
3.3 Dorsally Projecting Motorneurons: The RN2 and CQ Pattern
3.4 Characterisation of Motorneurons for A8/9 and their Targets
3.5 Characterisation of 3rd Instar Motorneurons in the Reference Segment
3.6 Discussion

4.) Relating Muscles and Neurons of A8/9 to those of A2-7
4.1 Introduction
4.2 Relating Muscles: Marker Expression Profiles
4.3 Relating Muscles: Development of S59 Positive Muscles
4.4 Relating Neurons: VT1, LT1 and the Role of the Transverse Nerve
4.5 Relating Neurons: aCC and RP2
4.5 Discussion

5.) Neuronal Cell Death as a Mean of Diversification
5.1 Introduction
5.2 No Indication of a Differentiating DA1 Provides a Model System
5.3 Cell-Death of Differentiated Motorneurons: aCC in A7 and RP2 in A8
5.4 Cas-3 Signal as Direct Evidence for Apoptosis
5.5 Survival of aCC in A7 and RP2 in A8 in a deficiency that removes grim, reaper and hid
5.6 Discussion

6.) Cues Underlying the Diversification
6.1 Introduction
6.2 Motorneurons aCC in A7 and RP2 in A8 survive in Abd-B Mutants
6.3 Abd-B Null Mutation affects the Formation of DA1
6.4 Abd-B Null Mutation disrupts the Muscle Pattern
6.5 Ectopic Expression of Abd-B leads to Cell Death in Specific Neurons
6.6 Ectopic Expression of Abd-B Does Not Prevent the Formation of DA1
6.7 Ectopic Expression of Abd-B changes the Morphology of VT1
6.8 Mapping the Hox-proteins in the CNS at 14 hrs AEL
6.9 Mapping Co-Factors of Hox Proteins in the CNS at 14 hrs AEL
6.10 Discussion
6.11 t-Test for the increased size of VT1 in AbdBM1 embryos

7.) Conclusions
7.1 Extending the Network: Arising Questions

8.) Material and Methods
8.1 Flystocks and Staging
8.2 Dechorionation and Wholemount Fixation
8.3 Immunohistochemisty
8.4 DAB Development
8.5 Dehydration and Mounting
8.6 Flat Preparations
8.7 Microscopy and Imaging
8.8 Dye Fills and Injections
8.9 Phalloidin Injections
8.10 Larval Preparations: Glue preparations and CNS Dissections
8.11 Heatshocks and Generation of MN-clones


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